What should I know when employed in a laboratory?

Consider blood and body fluids from all patients as infective. Refer to "Universal Precautions" for preventing transmission of bloodborne infectious diseases.

  • Report immediately a needlestick or sharp injury to a designated person because if post-exposure chemoprophylaxis is to be implemented, it should begin preferably within 1 to 2 hours after exposure.

What personal protection should I wear?

Wear gloves:

  • when processing blood or body fluid specimens
  • when handling contaminated articles
  • during cleaning and decontamination procedures

Change gloves after processing specimen.

Wear gowns:

  • while working with potentially infectious material

Wear masks and protective eye wear:

  • if mucous membrane contact is likely

Remove protective clothing before leaving laboratory.

Wash hands:

  • after processing specimens
  • after completing laboratory activities
  • after removing protective clothing
  • after a glove tear or suspected glove leak
  • before leaving laboratory

Do not pipette by mouth.

What precautions should I follow when working with needles and sharps?

  • Use needles only when there is no alternative. Consider needles and sharp instruments as potentially infective.
  • Use puncture-resistant containers. Label containers.
  • Clean and sterilize reusable syringes before reusing.
  • Do not recap, bend, break or remove needles by hand from disposable syringes or otherwise handle after use.

When should I decontaminate lab materials and surfaces?

Laboratory surfaces should be decontaminated:

  • After a spill of blood or body fluid. Disinfect with a 1:100-1:10 dilution of household bleach in water.
  • Before reprocessing contaminated material used in lab tests.
  • When work activities have been completed.

How should I dispose and transport contaminated lab test material?

  • Place contaminated lab test material in bags and dispose according to policy for infectious waste.
  • Put specimen into a sturdy container with secure lid to prevent leaking.
  • Avoid contaminating outside of container and accompanying lab form.
  • Place container in a clear, impervious plastic bag.

Document last updated on May 23, 2000